Open AccessComparison of a point-of-care serum amyloid A analyzer frequently used in equine practice with 2 turbidimetric immunoassays used in human and veterinary medicine
Author(s) -
J Kiemle,
Sarah Hindenberg,
Natali Bauer,
Michael Roecken
Publication year - 2021
Publication title -
journal of veterinary diagnostic investigation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.529
H-Index - 78
eISSN - 1943-4936
pISSN - 1040-6387
DOI - 10.1177/10406387211056029
Subject(s) - medicine , hum , serum amyloid a , point of care , bilirubin , pathology , art , performance art , inflammation , art history
Rapid, accurate detection of serum amyloid A (SAA) is needed in equine practice. We validated a patient-side point-of-care (POC) assay (Stablelab; Zoetis) compared to the turbidimetric immunoassays LZ-SAA (TIA-Hum) and VET-SAA (TIA-Vet; both Eiken Chemical). Analytical performance was assessed at 3 different concentration ranges and with interferences. Inter-method comparison using 49 equine serum samples revealed a significant difference between median SAA results ( p 3,000 mg/L for the POC assay. Equine serum SAA was stable over a median period of 2.5 y when stored at −80°C. Overall, there was excellent-to-moderate correlation between tests, but imprecision and hook effect of the POC, as well as bias between the methods, must be considered.