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Glutamine Reduces Phorbol‐12,13‐Dibutyrate‐Induced Macromolecular Hyperpermeability in HT‐29Cl.19A Intestinal Cells
Author(s) -
Kouznetsova Luda,
Bijlsma Pieter B.,
van Leeuwen Paul A. M.,
Groot Jack A.,
Houdijk Alexander P. J.
Publication year - 1999
Publication title -
journal of parenteral and enteral nutrition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.935
H-Index - 98
eISSN - 1941-2444
pISSN - 0148-6071
DOI - 10.1177/0148607199023003136
Subject(s) - glutamine , chemistry , macromolecule , biochemistry , amino acid
Background: Loss of mucosal integrity is associated with intestinal hyperpermeability, which may be inhibited by glutamine. The nature of this effect is unknown. The effect of glutamine on protein kinase C (PKC)‐mediated hyperpermeability in HT‐29Cl.19A intestinal cells was studied. Methods: Confluent monolayers ofHT‐29Cl.19A cells were cultured on permeable filters and mounted in Ussing chambers for permeability studies. Apical to basolateral transepithelial permeability for intact horseradish peroxidase (HRP) was determined. Phorbol‐12,13‐dibutyrate (PDB) was used to activate PKC‐mediated hyperpermeability, and the effect of glutamine (0.6 mmol/L) was studied. Results: Two hours of PDB stimulation increased the HRP flux, reaching five times control values after 4 hours. Bilateral exposure to glutamine for 4 hours reduced PDB‐induced hyperpermeability (37%). Preincubation with glutamine 2 hours before PDB stimulation showed an earlier and greater effect (3 hours, 43%; 4 hours, 50%). This bilateral effect of glutamine was mimicked by separate apical exposure. Basolateral exposure alone had no effect. Conclusions: Glutamine rapidly reduced the PKC‐mediated hyperpermeability for HRP in HT‐29Cl.19A intestinal cells. The dependency on apical exposure suggests that glutamine may be more effective when delivered by the enteral route. (Journal of Parenteral and Enteral Nutrition 23: 136–139, 1999)