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Alanyl‐Glutamine‐Supplemented Parenteral Nutrition Increases Luminal Mucus Gel and Decreases Permeability in the Rat Small Intestine
Author(s) -
Khan Jesmine,
Iiboshi Yasuhiko,
Cui Li,
Wasa Masafumi,
Sando Kinya,
Takagi Yoji,
Okada Akira
Publication year - 1999
Publication title -
journal of parenteral and enteral nutrition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.935
H-Index - 98
eISSN - 1941-2444
pISSN - 0148-6071
DOI - 10.1177/014860719902300124
Subject(s) - glutamine , crypt , jejunum , mucus , medicine , small intestine , intestinal permeability , gastroenterology , biology , intestinal mucosa , endocrinology , biochemistry , amino acid , ecology
Background: Effect of supplemental alanyl‐glutamine in standard TPN (S‐TPN) on luminal mucus gel and small intestinal permeability was investigated. Methods: Thirty Sprague‐Dawley rats were divided into group I (n = 10), receiving standard rat diet; group II (n = 10), receiving S‐TPN; and group III (n = 10), receiving alanyl‐glutamine‐supplemented TPN for 1 week. After 1 week, fluorescein isothiocyanate (FITC)‐dextran was injected into the small intestine of the rats, and they were killed. A small intestinal sample and portal blood were obtained for morphologic and functional analysis of mucus gel and intestinal permeability. Results: In group II, thickness and optical density of mucus gel per millimeter serosal length of intestine were significantly lower than group I (p <.001) and were significantly higher in group III than in group II ( p <.001). The number of goblet cells in the villi and in the crypt of the small intestine was significantly lower in group II than in group I (p <.001) and was significantly higher in group III than in group II (p <.001), with the exception of the villi of jejunum. Villous and crypt surface area per millimeter serosal length of intestine was significantly lower in group II than in group I (p <.001) and was significantly higher in group III than in group II (p <.001). Small intestinal permeability to FITC‐dextran was significantly higher in group II than in group I (p <.001) and was significantly lower in group III than in group II (p <.001). Glucosamine synthetase level was significantly higher in group III than in group I and ileum of group II (p <.001). Conclusions: Alanyl‐glutamine‐supplemented TPN prevents a decrease in mucus gel and an increase in small intestinal permeability associated with S‐TPN. (Journal of Parenteral and Enteral Nutrition 23: 24–31, 1999)