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Effects of Nucleosides and a Nucleotide Mixture on Gut Mucosal Barrier Function on Parenteral Nutrition in Rats
Author(s) -
Kishibuchi Masanori,
Tsujinaka Toshimasa,
Yano Masahiko,
Morimoto Takashi,
Jima Shohei,
Ogawa Atsuhiro,
Shiozaki Hitoshi,
Monden Morito
Publication year - 1997
Publication title -
journal of parenteral and enteral nutrition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.935
H-Index - 98
eISSN - 1941-2444
pISSN - 0148-6071
DOI - 10.1177/0148607197021002104
Subject(s) - barrier function , parenteral nutrition , lactulose , enteral administration , medicine , intestinal mucosa , ileum , gastroenterology , intestinal permeability , endocrinology , chemistry , biology , microbiology and biotechnology
Background: We have previously reported that the addition of nucleosides and a nucleotide mixture (OG‐VI) to total parenteral nutrition (TPN) prevents TPN‐induced intestinal mucosal atrophy and results in increased intestinal cathepsin activities under TPN. The aims of the present study are to examine the effects of OG‐VI on mucosal barrier functions and to clarify whether the intestinal cathepsins can be used as a new marker of mucosal barrier function. Method: Male Wistar rats were divided into three groups: FED (food ad libitum), TPN (a standard TPN solution), and OG (OG‐VI in addition to the TPN solution). Mucosal barrier functions were analyzed by the lactulose/mannitol (L/M) test, mucosal ZO‐1 messenger RNA level, and electron micrographs in the gut. Intestinal cathepsin B, H, and L activities were also measured. Results: The L/M ratio for TPN was much higher than that for FED. Only lactulose excretion was significantly reduced by OG‐VI. The intercellular spaces of the mucosal cells for TPN were significantly wider than those for FED, but were reduced in size by the addition of OG‐VI. All cathepsin activities of the ileum were higher for TPN than for FED, whereas OG showed no increase. Conclusion: OG‐VI contributes to the improvement of gut mucosal barrier function. Intestinal cathepsin activities can be used as a new marker of mucosal barrier function. (Journal of Parenteral and Enteral Nutrition 21: 104–111, 1997)