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Short‐Chain Fatty Acids Increase Proglucagon and Ornithine Decarboxylase Messenger RNAs After Intestinal Resection in Rats
Author(s) -
Tappenden Kelly A.,
Thomson Alan B.R.,
Wild Gary E.,
McBurney Michael I.
Publication year - 1996
Publication title -
journal of parenteral and enteral nutrition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.935
H-Index - 98
eISSN - 1941-2444
pISSN - 0148-6071
DOI - 10.1177/0148607196020005357
Subject(s) - proglucagon , messenger rna , ornithine decarboxylase , chemistry , medicine , ornithine decarboxylase antizyme , endocrinology , biochemistry , biology , enzyme , gene , glucagon like peptide 1 , type 2 diabetes , diabetes mellitus
Background: Intestinal adaptation is a complex physiological process that is not completely understood. Systemic administration of short‐chain fatty acids (SCFAs) has been shown to facilitate adaptation to small bowel resection; however the mechanisms underlying this phenomena are unknown. Methods: Forty‐six male Sprague‐Dawley rats underwent an 80% jejunoileal resection and jugular catheterization. After surgery, rats were randomly assigned to receive standard total parenteral nutrition (TPN) or an isoenergetic, isonitrogenous TPN supplemented with SCFAs. On day 3 or 7 after surgery, ileal samples were removed for determination of mucosal wet weight, DNA, RNA, and protein concentrations. Total cellular RNA was extracted for use in Northern blot analysis to quantify proglucagon and ornithine decarboxylase messenger RNAs (mRNAs). Results: Total, mucosal, and submucosal weights were increased (p <.05) in the SCFA group both 3 and 7 days after surgery. Ileal DNA and RNA concentrations were increased (p <.05) in the SCFA group at both time points; however ileal protein concentration did not differ between groups until 7 days after resection. Levels of proglucagon and ornithine decarboxylase messenger RNAs were higher (p <.05) in the SCFA group at both time points. Conclusion: The upregulation of proglucagon and ornithine decarboxylase gene expression may be the mechanism by which SCFAs facilitate intestinal adaptation. (Journal of Parenteral and Enteral Nutrition 20 :357–362, 1996)