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Interrelation of Intracellular Proteases With Total Parenteral Nutrition‐Induced Gut Mucosal Atrophy and Increase of Mucosal Macromolecular Transmission in Rats
Author(s) -
Kishibuchi Masanori,
Tsujinaka Toshimasa,
Jima Shohei,
Yano Masahiko,
Ebisui Chikara,
Kan Kazuomi,
Morimoto Takashi,
Mori Takesada
Publication year - 1995
Publication title -
journal of parenteral and enteral nutrition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.935
H-Index - 98
eISSN - 1941-2444
pISSN - 0148-6071
DOI - 10.1177/0148607195019003187
Subject(s) - ileum , jejunum , parenteral nutrition , proteases , cathepsin , cathepsin b , medicine , intestinal mucosa , enteral administration , small intestine , barrier function , endocrinology , cathepsin l , cecum , biology , chemistry , biochemistry , enzyme , microbiology and biotechnology
Total parenteral nutrition (TPN) is known to induce mucosal atrophy and to increase macromolecular transmission of the small intestine. The potential participation of various proteases in that process was investigated. Male Wistar rats were randomly divided into two groups: the TPN group (n = 11) received a standard TPN (250 kcal/kg per day, 1.78 g nitrogen/kg per day) and the FED group (n = 10) received a standard rat food for 1 week. This was followed by an examination of gut macromolecular transmission of fluorescein isothiocyanate dextran 70,000 (FITC‐dextran) after intragastric injection and of the activities of gut mucosal cathepsins B, H, and L and of proteasome. Mucosal wet weight and protein content decreased significantly by TPN for 1 week. In both groups, the activities of all proteases in the ileum were significantly greater than in the jejunum. In the TPN group, cathepsin L and H activities in the ileum, and cathepsin B activity in both the jejunum and the ileum, were greater than those in the FED group. The portal concentration of FITC‐dextran was higher than arterial and venous concentrations in the both groups. In the TPN group, the portal FITC‐dextran concentration increased significantly compared with the FED group. In conclusion, active proteolysis is not associated with TPN‐induced mucosal atrophy. Cathepsins activities in the ileum increase as a result of TPN. Interrelationship is implicated between increase of lysosomal protease activity and the deterioration of the intestinal barrier function, which permits macromolecular transmission. ( Journal of Parenteral and Enteral Nutrition 19: 187–192, 1995)