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Arginine‐Enriched Solution Induces a Marked Increase in Muscle Glutamine Concentration and Enhances Muscle Protein Synthesis in Tumor‐Bearing Rats
Author(s) -
Oka Toshinori,
Ohwada Katsuo,
Nagao Mitsuhiro,
Kitazato Kenji,
Kishino Yasuo
Publication year - 1994
Publication title -
journal of parenteral and enteral nutrition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.935
H-Index - 98
eISSN - 1941-2444
pISSN - 0148-6071
DOI - 10.1177/0148607194018006491
Subject(s) - glutamine , arginine , leucine , valine , amino acid , isoleucine , medicine , skeletal muscle , glycine , endocrinology , parenteral nutrition , ornithine , protein biosynthesis , biology , biochemistry , chemistry
Using a transplantable Yoshida sarcoma in a rat model of total parenteral nutrition (TPN), we measured the effectiveness of an arginine‐enriched amino acid solution (AI‐82) on muscle glutamine concentration and muscle protein synthesis compared with that of a conventional amino acid solution (Proteaminl2). After tumor‐bearing rats had been given one of two isocaloric TPN regimens for 6 days, [ 15 N]glycine (99 atom %) containing TPN solution was infused into animals at a constant rate of 8 mg of [ 15 N]glycine per hour for 18 hours, after which the liver, skeletal muscle (gastrocnemius muscle), and tumor protein synthesis rates were measured. A significantly increased whole muscle protein synthesis rate was observed in the AI‐82 group; there was no difference in the whole liver and tumor protein synthesis rates between the two groups. When each TPN solution was administered for 1 week, muscle concentrations of arginine, ornithine, glutamine, and glutamate were considerably higher in the AI‐82 group than in the Proteamin12 group, and these differences were also accompanied by a decrease in the plasma branched‐chain amino acid (BCAA) (leucine, isoleucine, and valine) levels in the AI‐82 group. The high levels of muscle glutamine concentration in the AI‐82 group were investigated in connection with the high use of exogenous branched‐chain amino acids. ( Journal of Parenteral and Enteral Nutrition 18: 491–496, 1994)