Premium
Effect of Methionine‐Deprived Nutrition on Cell Growth and Cell Kinetics in Cell Cultures and Experimental Tumors
Author(s) -
Usami Makoto,
Ohyanagi Harumasa,
Ishimoto Sachiko,
Nishimatsu Shinichi,
Ueda Tomoki,
Saitoh Yoichi
Publication year - 1991
Publication title -
journal of parenteral and enteral nutrition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.935
H-Index - 98
eISSN - 1941-2444
pISSN - 0148-6071
DOI - 10.1177/0148607191015005540
Subject(s) - methionine , cell growth , cell cycle , dna synthesis , cell , bromodeoxyuridine , cell culture , flow cytometry , kinetics , cell division , biology , biochemistry , in vitro , chemistry , microbiology and biotechnology , amino acid , genetics , physics , quantum mechanics
The effect of methionine‐deprived nutrition on cell growth and cell kinetics was investigated in cell cultures and in tumor‐bearing rats using the total parenteral nutrition (TPN) technique. A simultaneous flow cytometric measurement of the cellular DNA content and the amount of 5‐bromodeoxyuridine incorporated into cellular DNA was performed for analysis of cell kinetics. The methionine‐free medium demonstrated a cytocidal effect on the growth of SLC cells after 6 hours of culturing. It decreased viability from 80% in the control medium to 23%, and it decreased the S phase and increased the G 0 /G 1 phase of the cell cycles. The methionine‐deprived medium showed a concentration‐dependent inhibition in cellular growth. Methionine‐deprived TPN was seen to inhibit AH109A and SLC tumor growth compared with conventional TPN and decreased the S phase and increased the Go/ G 1 phase of cell cycles. These results confirm that methionine deprivation blocks cells from processing into the G 1 phase and recycling, and that it is effective in inhibiting tumor growth in cultures and in vivo. ( Journal of Parenteral and Enteral Nutrition 15: 540–545, 1991)