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Comparative Effects of Thermal and Surgical Trauma on Rat Muscle Protein Metabolism
Author(s) -
Lee Mermel Virginia,
Wolfe Bruce M.,
Hansen Robert J.,
Clifford Andrew J.
Publication year - 1991
Publication title -
journal of parenteral and enteral nutrition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.935
H-Index - 98
eISSN - 1941-2444
pISSN - 0148-6071
DOI - 10.1177/0148607191015002128
Subject(s) - myofibril , tyrosine , sarcoplasm , muscle protein , protein turnover , intracellular , protein degradation , medicine , gastrocnemius muscle , parenteral nutrition , endocrinology , protein biosynthesis , enteral administration , chemistry , protein metabolism , metabolism , biochemistry , skeletal muscle , calcium
A modified intraperitoneal pool flooding technique, employing L‐ 3 H‐tyrosine, was developed for measuring muscle protein synthetic rates following traumatic injury. Sufficient radiolabeled tyrosine was injected intraperitoneally to effect a six‐fold increase in plasma tyrosine concentration (124–800 μ M) resulting in constant, sustained specific radioactivities in plasma‐ and intracellular‐free tyrosine pools. Localized vs systemic effects of thermal and surgical trauma on gastrocnemius muscle protein turnover were assessed 2 and 4 days postinjury. Thermal trauma increased total, myofibrillar, and sarcoplasmic muscle protein synthesis (44%) and protein degradation (300%). Conversely, surgical trauma decreased synthesis of total (24%), myofibrillar (14%), and sarcoplasmic (43%) muscle proteins without altering protein degradation. Short‐term restriction of pair‐fed controls did not affect either aspect of protein turnover. (Journal of Parenteral and Enteral Nutrition 15 :128–136, 1991)