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Effect of Emulsions of Medium and Long Chain Triglyceride on Human Adipose Tissue Prostaglandin Production in Vitro
Author(s) -
Katz David P.,
Rudick Jack,
Knittle Jerome L.
Publication year - 1988
Publication title -
journal of parenteral and enteral nutrition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.935
H-Index - 98
eISSN - 1941-2444
pISSN - 0148-6071
DOI - 10.1177/0148607188012002178
Subject(s) - adipose tissue , triglyceride , endocrinology , medicine , chemistry , prostaglandin , phospholipid , white adipose tissue , fatty acid , prostacyclin , arachidonic acid , biochemistry , biology , cholesterol , membrane , enzyme
Preliminary in vitro studies have been performed to assess the effect of experimental lipid emulsions of varying fatty acid composition on human adipose tissue metabolism. Subcutaneous human adipose tissue was obtained during elective surgery and placed in tissue culture. Physical mixtures of long chain triglyceride (LCT) and/or medium chain triglyceride (MCT) were added to the tissue culture medium so that the final concentration was 400 mg/dl. After a 3‐day incubation period the tissue was harvested, placed in buffer and used to determine in vitro production of the prostaglandins prostacyclin I 2 (measured as its stable endproduct 6‐keto PGF 1α ), thromboxane A 2 (measured as TXB 2 ), and prostaglandin E 2 . Measurements of the fatty acid profile found in the neutral‐ and phospholipid fraction of the adipose tissue and fat cell size were also made. The results demonstrate that samples incubated in 100% MCT had the most significant increase in prostaglandin production whereas those incubated in 100% LCT had the most significant decrease in activity of the three prostaglandins assayed, when compared to controls. The addition of LCT to MCT caused a dose‐related decrease in adipose tissue prostaglandin production. There were no significant changes in the profile of fatty acids found in the neutral‐ or phospholipid fraction of adipose tissue. The results indicate that the relative level of MCT/LCT incubated with human adipose tissue has a significant effect on prostaglandin production. ( Journal of Parenteral and Enteral Nutrition 12 :178–184, 1988)