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Microbial Growth Patterns in a Total Parenteral Nutrition Formulation Containing Lipid Emulsion
Author(s) -
Gilbert Michael,
Gallagher S. Cathy,
Eads Michael,
Elmore Michael F.
Publication year - 1986
Publication title -
journal of parenteral and enteral nutrition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.935
H-Index - 98
eISSN - 1941-2444
pISSN - 0148-6071
DOI - 10.1177/0148607186010005494
Subject(s) - parenteral nutrition , emulsion , lipid emulsion , fat emulsion , chemistry , food science , medicine , intensive care medicine , biochemistry
Microbial growth of Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa and Candida albicans was evaluated in a standard amino acid‐dextrose‐based total parenteral nutrition (TPN) solution, 10% lipid emulsion, and a combined TPN formulation containing amino acids, dextrose, and lipid emulsion. At an initial inoculum of 10 4 CFU/ml, all three bacterial organisms grew well in 10% lipid emulsion, died in the standard solution and grew only minimally or died in the combined formulation. C. albicans grew in all three formulations at an initial inoculum of 10 4 CFU/ml; however, at an initial inoculum of 10 2 CFU/ml, which approximates touch contamination, growth of Candida in the standard and combined formulations was <1 log at 24 hr in contrast to the 10% lipid emulsion which showed significant growth >2 log at 24 hr. It is concluded that a 24‐hr infusion time is safe for the combined TPN formulation used in this study. This should result in significant cost savings compared to the previously recommended 12‐hr infusion time. ( Journal of Parenteral and Enteral Nutrition 10: 494–497, 1986)