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Amino Acids and Indoleamines in the Brain after Infusion of Branched‐Chain Amino Acids to Rats with Liver Ischemia
Author(s) -
Bugge M.,
Bengtsson F.,
Nobin A.,
Holmin T.,
Jeppsson B.,
Hultberg B.,
Falck B.,
Herlin P.
Publication year - 1986
Publication title -
journal of parenteral and enteral nutrition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.935
H-Index - 98
eISSN - 1941-2444
pISSN - 0148-6071
DOI - 10.1177/0148607186010005474
Subject(s) - phenylalanine , glutamine , valine , medicine , endocrinology , methionine , amino acid , isoleucine , chemistry , leucine , alanine , biochemistry
Rats with a portacaval anastomosis and ligation of the hepatic artery 2 days later were infused for 6 hr with a 10% glucose solution (group I) or the same solution combined with 0.24 M/liter branched‐chain amino acids (BCAA, group II). Control animals with portacaval anastomosis and sham‐operation (group III) or two sham‐operations (group IV) were infused with a 10% glucose solution. The rats were killed by decapitation and indoleamines and amino acids were determined in the brain. Rats with liver ischemia were stuporous at the end of the experiment irrespective of treatment. The concentrations in the cortex of lysine, methionine, phenylalanine, threonine, alanine, glutamine, glycine, histidine, and tyrosine were significantly increased in group I compared to group IV. Infusion of BCAA to rats with liver‐ischemia (group II) resulted in significantly lower concentrations of lysine, methionine, phenylalanine, threonine, histidine and tyrosine and increased concentrations of isoleucine, leucine, valine, and arginine compared to group I. The content of serotonin in the cortex and brain stem was significantly increased in group I compared with the BCAAtreated animals (group II) and the control groups III and IV. The concentrations of 5‐hydroxyindoleacetic acid (5‐HIAA) in the cortex and brain stem were higher in group I than in group IV. Infusion of BCAA to rats with liver ischemia normalized the concentrations of 5‐HIAA in the cortex and brain stem. (Journal of Parenteral and Enteral Nutrition 10:474–478,1986)