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Lack of Modulation of Postoperative Immunosuppression by Isotonic Amino Acid Infusion
Author(s) -
Neuvonen P.,
Salo M.,
Perttilä J.,
Havia T.
Publication year - 1986
Publication title -
journal of parenteral and enteral nutrition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.935
H-Index - 98
eISSN - 1941-2444
pISSN - 0148-6071
DOI - 10.1177/0148607186010002160
Subject(s) - pokeweed mitogen , concanavalin a , lymphocyte , immunosuppression , immune system , enteral administration , medicine , antibody , immunology , parenteral nutrition , endocrinology , chemistry , in vitro , biochemistry
Effects of an isotonic amino acid solution on the immune defence systems of surgical patients were studied. Twenty‐two patients admitted for major abdominal operations were randomly allocated into two groups. Eleven of them received 1 liter of 3% amino acid solution daily for 5 days postoperatively, while 11 control patients received balanced 5% glucose‐containing electrolyte solutions. Immune variables were studied preoperatively and at 1, 3 to 4, and 6 to 7 days postoperatively. These studies included leukocyte and differential counts, T (OKT3+), B (sIg+), T helper/inducer (OKT4+) and suppressor/cytotoxic (OKT8+) cell determinations, measurement of in vitro lymphocytic responses to phytohemagglutinin (PHA), pokeweed mitogen (PWM) and Staphylococcus aureus Cowan I (StaCw) in cultures of separated lymphocytes, and to PHA, concanavalin A (Con A), and PWM in whole blood cultures. B lymphocyte functions were studied by measuring synthesis of immunoglobulins IgG, IgM, and IgA in the cell culture medium when lymphocytes were stimulated with PWM alone and in the presence of Con A or hydrocortisone. Postoperative amino acid infusion slightly improved the capacity of lymphocytes to synthetize and secrete IgG and IgM. By contrast, no improvement was seen in cell counts or lymphocyte transformation patterns. (Journal of Parenteral and Enteral Nutrition 10: 160–165, 1986)