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Microbial Growth Comparisons of Five Commercial Parenteral Lipid Emulsions
Author(s) -
Crocker Kathleen S.,
Noga Richard,
Filibeck Donald J.,
Krey Susanna H.,
Markovic Mario,
Steffee William P.
Publication year - 1984
Publication title -
journal of parenteral and enteral nutrition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.935
H-Index - 98
eISSN - 1941-2444
pISSN - 0148-6071
DOI - 10.1177/0148607184008004391
Subject(s) - parenteral nutrition , lipid emulsion , medicine , intensive care medicine , food science , chemistry
The ability of parenteral lipid emulsions to support microbial growth was compared using commercially available brands of lipid emulsion. Both 10 and 20% concentrations of soybean and safflower oil emulsions were used. Washed cultures of six gram‐negative, three gram‐positive, and one yeast, in concentrations of 1 × 10 4 to 2 × 10 4 colony‐forming units/ml, were inoculated into lipid emulsion aliquots and stored at room temperature. These were then subcultured at 0, 6, 12, 24 and 48 hr. After 48 hr at 37°C, growth was recorded as colony‐forming units/ml. Normalized growth curves were expressed as x ± SEM. ANOVA demonstrated no difference in growth patterns due to the nature of the oil or its concentration. Gram‐negative organisms multiplied faster when compared to gram‐positive ( p < 0.05 at 12 hr, p < 0.01 at 24 hr, and p < 0.005 at 48 hr). Yeast grew as well as bacteria. The Center for Disease Control's recommendation of a 12‐hr hang time for parenteral lipid emulsions should be observed until correlation of laboratory microbial growth patterns and clinical use are studied further. ( Journal of Parenteral and Enteral Nutrition 8 :391–395, 1984)