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Rapid Determination of Cold Insoluble Globulin by Laser Nephelometry. Application in Patients Receiving Preoperative Total Parenteral Nutrition
Author(s) -
Brodin Bengt,
Cederblad Gitten,
Larsson Jörgen,
Schildt Bo,
Sjödahl Rune,
Symreng Tommy,
Wetterfors Jarl
Publication year - 1982
Publication title -
journal of parenteral and enteral nutrition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.935
H-Index - 98
eISSN - 1941-2444
pISSN - 0148-6071
DOI - 10.1177/0148607182006003214
Subject(s) - nephelometry , parenteral nutrition , orosomucoid , medicine , haptoglobin , transthyretin , albumin , gastroenterology , acute phase protein , globulin , transferrin , ceruloplasmin , immunology , chemistry , inflammation , glycoprotein , biochemistry , antibody
A simple and rapid method for determining plasma fibronectin or cold insoluble globulin (CIG) based on immunochemical precipitation and laser nephelometry is described. The coefficient of variation within the series was 4.2% and between the series 9.1%. The mean value for 37 control subjects was 100.1% ± 20.6 (SD). Nineteen patients with gastrointestinal carcinoma or Crohn's disease were investigated on admission. The mean value of their plasma CIG was 104.7 ± 26.9 (SD), which was not statistically different from the control subjects. Twelve of the patients received total parenteral nutrition (TPN) during the two preoperative weeks. The concentration of CIG was significantly increased after one and two weeks of TPN compared to the initial value. Six out of seven patients that postoperatively showed signs of infection had CIG values below 90% on admission. Of several other plasma proteins determined on admission, only a statistically significantly negative relationship to transferrin was found. CIG did not significantly relate either to the acute phase reactants, haptoglobin and orosomucoid, or to the visceral proteins albumin, choline esterase or prealbumin.