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Partial Replacement of ω‐6 Fatty Acids With Medium‐Chain Triglycerides, but Not Olive Oil, Improves Colon Cytokine Response and Damage in Experimental Colitis
Author(s) -
Bertevello Pedro L.,
De Nardi Leticia,
Torrinhas Raquel S.,
Logullo Angela F.,
Waitzberg Dan L.
Publication year - 2012
Publication title -
journal of parenteral and enteral nutrition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.935
H-Index - 98
eISSN - 1941-2444
pISSN - 0148-6071
DOI - 10.1177/0148607111421788
Subject(s) - medicine , colitis , soybean oil , gastroenterology , tumor necrosis factor alpha , ulcerative colitis , cytokine , necrosis , parenteral nutrition , saline , fatty acid , histology , chemistry , pathology , biochemistry , disease
Background : Soybean oil is rich in ω‐6 fatty acids, which are associated with higher incidence and more severe cases of inflammatory bowel diseases. The authors evaluated whether partial replacement of soybean oil by medium‐chain triglycerides (MCTs) or olive oil influenced the incidence and severity of experimental ulcerative colitis by using different parenteral lipid emulsions (LEs). Methods : Wistar rats (n = 40) were randomized to receive parenteral infusion of the following LE: 100% soybean oil (SO), 50% MCT mixed with 50% soybean oil (MCT/SO), 80% olive oil mixed with 20% soybean oil (OO/SO), or saline (CC). After 72 hours of infusion, acetic acid experimental colitis was induced. After 24 hours, colon histology and cytokine expression were analyzed. Results : SO was not significantly associated with overall tissue damage. MCT/SO was not associated with necrosis ( P < .005), whereas OO/SO had higher frequencies of ulcer and necrosis ( P < .005). SO was associated with increased expression of interferon‐γ ( P = .005) and OO/SO with increased interleukin (IL)–6 and decreased tumor necrosis factor–α expression ( P < .05). MCT/SO appeared to decrease IL‐1 ( P < .05) and increase IL‐4 ( P < .001) expression. Conclusions : Parenteral SO with high concentration of ω‐6 fatty acids was not associated with greater tissue damage in experimental colitis. SO partial replacement with MCT/SO decreased the frequency of histological necrosis and favorably modulated cytokine expression in the colon; however, replacement with OO/SO had unfavorable effects.