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A New Enteral Diet, MHN‐02, Which Contains Abundant Antioxidants and Whey Peptide, Protects Against Carbon Tetrachloride–Induced Hepatitis
Author(s) -
Takayanagi Takehiko,
Sasaki Hajime,
Kawashima Akihiro,
Mizuochi Yuichiro,
Hirate Hiroyuki,
Sugiura Takeshi,
Azami Takafumi,
Asai Kiyofumi,
Sobue Kazuya
Publication year - 2011
Publication title -
journal of parenteral and enteral nutrition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.935
H-Index - 98
eISSN - 1941-2444
pISSN - 0148-6071
DOI - 10.1177/0148607110381599
Subject(s) - medicine , oxidative stress , superoxide dismutase , carbon tetrachloride , enteral administration , hepatitis , lactate dehydrogenase , endocrinology , pharmacology , biochemistry , chemistry , parenteral nutrition , enzyme , organic chemistry
Background : Inflammatory or oxidative stress is related to various diseases, including not only inflammatory diseases, but also diabetes, cancer, and atherosclerosis. The aim of this study was to evaluate the anti‐inflammatory effects of a new enteral diet, MHN‐02, which contains abundant antioxidants and whey peptide. The study also investigated the ability of MHN‐02 to attenuate lethality, liver injury, the production of inflammatory cytokines, and the production of oxidized products using a carbon tetrachloride–induced rat model of severe fulminant hepatitis. Methods : Male Sprague‐Dawley rats were fed either a control diet or the MHN‐02 diet for 14 days and injected with 2 mL/kg of carbon tetrachloride. Survival of rats was monitored from day 0 to day 3. To evaluate liver injury, inflammation, and oxidative stress, blood and liver samples were collected, and aspartate aminotransferase, alanine aminotransferase, lactate dehydrogenase, interleukin 6, tumor necrosis factor–α, and superoxide dismutase activity as a free radical scavenger were measured. A portion of the liver was evaluated histologically. Results : The survival rates of rats receiving the MHN‐02 diet and the control diet were 90% and 55%, respectively. In the MHN‐02 diet group, levels of serum liver enzymes and serum cytokines were significantly lower than in the control group. Superoxide dismutase activity in the MHN‐02 diet was significantly higher in the MHN‐02 group. Pathological lesions were significantly larger in the control group. Conclusion : Supplementation of enteral diets containing whey peptide and antioxidants may protect against severe hepatitis.