Premium
Carbohydrate Supplementation Before Operation Retains Intestinal Barrier Function and Lowers Bacterial Translocation in a Rat Model of Major Abdominal Surgery
Author(s) -
Bouritius Hetty,
Hoorn Danny C.,
Oosting Annemarie,
MiddelaarVoskuilen Mariska C.,
Limpt Cees J. P.,
Lamb Kelly J.,
Leeuwen Paul A. M.,
Vriesema Aldwin J. M.,
Norren Klaske
Publication year - 2008
Publication title -
journal of parenteral and enteral nutrition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.935
H-Index - 98
eISSN - 1941-2444
pISSN - 0148-6071
DOI - 10.1177/0148607108316191
Subject(s) - barrier function , carbohydrate , bacterial translocation , ussing chamber , ileum , mesenteric lymph nodes , intestinal permeability , ischemia , chromosomal translocation , anaerobic bacteria , anaerobic exercise , intestinal mucosa , medicine , gastroenterology , biology , endocrinology , physiology , spleen , bacteria , biochemistry , secretion , gene , microbiology and biotechnology , genetics
Background : Overnight fasting of rats augments the susceptibility of the small intestine to ischemia–reperfusion damage. Feeding before surgery may improve injuries to distant organs that were induced by ischemia–reperfusion. The present study tested the hypothesis that one of the food constituents, namely carbohydrates, may be responsible for the protective effect of preoperative feeding on postoperative organ dysfunction. Methods: Male Wistar rats were fed ad libitum for 5 d and had either free access to water or free access to a carbohydrate drink and water. Then they were fasted for 16 h and access remained to either water or a carbohydrate drink and water. Following this, the arteria mesenterica superior was clamped for 60 min followed by 180 min of reperfusion. Subsequently, the intestinal permeability of stripped ileum was determined by measuring the mucosal to serosal flux in Ussing chambers. For assessment of bacterial content, organs were aseptically removed and assessed for bacterial content by culture under anaerobic conditions. Results: Preoperative supplementation with carbohydrates resulted in a better maintenance of intestinal barrier function when compared with water supplemented animals. Moreover, carbohydrate supplementation resulted in a reduction in the ischemiareperfusion–induced increase in bacterial content of the liver, kidney, and mesenteric lymph nodes. Conclusions: Preoperative intake of carbohydrates by rats retains both the intestinal barrier function and prevents translocation of bacteria to distant organs.