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Intestinal Polymeric Immunoglobulin Receptor Is Affected by Type and Route of Nutrition
Author(s) -
Sano Yoshifumi,
Gomez F. Enrique,
Kang Woodae,
Lan Jinggang,
Maeshima Yoshinori,
Hermsen Joshua L.,
Ueno Chikara,
Kudsk Kenneth A.
Publication year - 2007
Publication title -
journal of parenteral and enteral nutrition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.935
H-Index - 98
eISSN - 1941-2444
pISSN - 0148-6071
DOI - 10.1177/0148607107031005351
Subject(s) - polymeric immunoglobulin receptor , lamina propria , immunoglobulin a , secretory component , enteral administration , antibody , receptor , medicine , biology , intestinal mucosa , endocrinology , chemistry , immunology , parenteral nutrition , immunoglobulin g , epithelium , genetics
Background: Secretory immunoglobulin A (SIgA) prevents adherence of pathogens at mucosal surfaces to prevent invasive infection. Polymeric immunoglobulin receptor (pIgR) is located on the basolateral surface of epithelial cells and binds dimeric immunoglobulin A (IgA) produced by plasma cells in the lamina propria. This IgA‐pIgR complex is transported apically, where IgA is exocytosed as SIgA to the mucosal surface. Our prior work shows that mice fed intragastric (IG, an elemental diet model) and IV parenteral nutrition (PN) solution have reduced intestinal T and B cells, SIgA, and interleukin‐4 (IL‐4) compared with mice fed chow or a complex enteral diet (CED). Prior work also demonstrates a reduction in IgA transport to mucosal surfaces in IV PN–fed mice. Because IL‐4 up‐regulates pIgR production, this work studies the effects of these diets on intestinal pIgR. Methods: Male Institute of Cancer Research (ICR) mice were randomized to chow (n = 11) with IV catheter, CED (n = 10) or IG PN (n = 11) via gastrostomy and IV PN (n = 12) for 5 days. CED and PN were isocaloric and isonitrogenous. Small intestine was harvested for pIgR and IL‐4 assays after mucosal washing for IgA. IgA and IL‐4 levels were analyzed by enzyme‐linked immunosorbent assay and pIgR by Western blot. Results: Small intestinal pIgR expression, IgA levels, and IL‐4 levels decreased significantly in IV PN and IG PN groups. Conclusions: Lack of enteral stimulation affects multiple mechanisms responsible for decreased intestinal SIgA levels, including reduced T and B cells in the lamina propria, reduced Th‐2 IgA‐stimulating cytokines, and impaired expression of the IgA transport protein, pIgR.