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Parenteral Nutrition and Fasting Reduces Mucosal Addressin Cellular Adhesion Molecule‐1 (MAdCAM‐1) mRNA in Peyer's Patches of Mice
Author(s) -
Gomez F. Enrique,
Lan Jinggang,
Kang Woodae,
Ueno Chikara,
Kudsk Kenneth A.
Publication year - 2007
Publication title -
journal of parenteral and enteral nutrition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.935
H-Index - 98
eISSN - 1941-2444
pISSN - 0148-6071
DOI - 10.1177/014860710703100147
Subject(s) - addressin , endocrinology , medicine , messenger rna , cell adhesion molecule , immune system , biology , immunology , andrology , receptor , gene , biochemistry , integrin
Background: Mucosal addressin cellular adhesion molecule‐1 (MAdCAM‐1) in Peyer's patches (PP) is the gateway molecule for cellular migration into the mucosal immune system. Lack of enteral feeding during parenteral nutrition (PN) rapidly decreases PP MAdCAM‐1, leading to drops in mucosal T and B cells and intestinal and respiratory IgA. We determined the molecular events associated with MAdCAM‐1 mRNA and protein during PN (short and long term) and fasting (1 and 2 days). Methods: Experiment 1: Cannulated mice received PN for 8 hours (short‐term PN, n = 6) or chow + saline (chow, n = 6). Experiment 2: Cannulated mice received PN (long‐term PN, n = 4) or chow (n = 3) for 5 days. Experiment 3: Noncannulated chow mice were fasted for 1 and 2 days (n = 2/time). Total cellular RNA from the PP was quantified for MAdCAM‐1 mRNA by real‐time polymerase chain reaction (PCR). MAdCAM‐1 protein was measured by Western blot. Results: PN rapidly down‐regulated MAdCAM‐1 gene expression. After 8 hours of PN with lack of enteral feeding, MAdCAM‐1 mRNA levels dropped 20% (0.8‐fold vs chow, p > .05); 5 days of PN reduced MAd‐CAM‐1 levels 64% (0.34‐fold vs chow, p < .05). PN reduced MAdCAM‐1 protein levels by 30% (chow: 329 ± 14 vs PN: 230 ± 35, p < .05) after 5 days. Fasting of uncannulated mice decreased MAdCAM‐1 mRNA levels by 16% (0.84‐fold, p < .05) at day 1 and 30% (0.7‐fold, p < .05) by day 2 compared with chow. Conclusions: Both PN with lack of enteral feeding and fasting down‐regulate MAdCAM‐1 mRNA and protein levels in PP. The MAdCAM‐1 changes are due to lack of enteral stimulation rather than toxic effects of PN.