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Effect of Energy Substrates on Protein Degradation in Isolated Small Intestinal Enterocytes From Rats
Author(s) -
Saberi Mohsen T.,
Stewart Sarah A.,
Annette Myriam,
Knowles Andrew L.,
Attaix Didier,
Samuels Sue E.
Publication year - 2006
Publication title -
journal of parenteral and enteral nutrition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.935
H-Index - 98
eISSN - 1941-2444
pISSN - 0148-6071
DOI - 10.1177/0148607106030006497
Subject(s) - protein degradation , butyrate , degradation (telecommunications) , glutamine , osmotic concentration , protein catabolism , enterocyte , biochemistry , catabolism , medicine , chemistry , biology , endocrinology , metabolism , small intestine , amino acid , fermentation , telecommunications , computer science
Background: Nutrients affect small intestinal protein mass and metabolism, but studies on the effect of nutrients on small intestinal protein degradation are very limited due to a lack of a proper method. The objectives of this study were to establish a method to directly estimate protein degradation in isolated enterocytes from rats and to test the effect of energy substrates on protein degradation. Methods: Male Sprague‐Dawley rats (150–200 g, n ≥ 8 per treatment) were used. Cell viability, tyrosine release as an indicator of protein degradation, and the effect of osmolarity, 50 mmol/L glucose, 20 mmol/L β‐hydroxybutyrate, 4.7 mmol/L butyrate, and 30 mmol/L glutamine on protein degradation were measured. Results: The average viability of enterocytes at time 30 minutes was 85.8% (range, 81%–94%). Tyrosine release was linear over the course of experiments, indicating constant protein degradation ( R 2 = 0.9943; p < .05). Osmolarity, glucose, and glutamine had no effect on protein degradation in isolated enterocytes. β‐Hydroxybutyrate significantly decreased it (–16%; p < .05), whereas butyrate slightly increased it (+5%; p < .05). Conclusions: A high viability and constant protein degradation indicate a successful establishment of a method to estimate protein degradation in isolated small intestinal enterocytes from rats. The large effect of β‐hydroxybutyrate suggests a potential positive role for ketone bodies to limit the loss of small intestinal protein mass by decreasing protein degradation.