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Assessment of Ascorbic Acid Stability in Different Multilayered Parenteral Nutrition Bags: Critical Influence of the Bag Wall Material
Author(s) -
Dupertuis Yves M.,
Ramseyer Sihan,
Fathi Marc,
Pichard Claude
Publication year - 2005
Publication title -
journal of parenteral and enteral nutrition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.935
H-Index - 98
eISSN - 1941-2444
pISSN - 0148-6071
DOI - 10.1177/0148607105029002125
Subject(s) - ascorbic acid , chemistry , chromatography , chemical stability , food science , organic chemistry
Background: The recent development of multilayered bags has minimized ascorbic acid oxidation in parenteral nutrition (PN) admixtures. However, the gas‐barrier property of multilayered bags depends on their plastic material. This study compared ascorbic acid stability in different multilayered bags under experimental conditions. Methods: Oxygen permeability of a newly developed 6‐layered bag (6‐L) was compared with a highly mechanical‐resistant 3‐layered bag (3‐L R ) and a highly flexible 3‐layered bag (3‐L F ) using gas chromatography. Ascorbic acid stability was assessed by iodine titration in bags filled with 2.5 L H 2 O and 40 g carbohydrates after setting residual O 2 content at ≤1 or ≥5 ppm. The effect of storage at 4°C, 21°C, and 40°C on ascorbic acid stability was assessed over 48 hours in a complete PN admixture (ie, 330 g carbohydrates, 100 g lipids, 96 g amino acids and trace elements) using high‐pressure liquid chromatography. Results: Oxygen permeability was markedly reduced in 6‐L bags (0.5 mL O 2 /m 2 /d) compared with 3‐L R (150 mL O 2 /m 2 /d) and 3‐L R (1500 mL O 2 /m 2 /d). Accordingly, ascorbic acid was more stable in 6‐L bags (half‐life [T 1/2 ] = 16 days up to 40°C) than in 3‐L R (T 1/2 = 9 days at 4°C, 47 hours at 21°C and 29 hours at 40°C) and 3‐L F (T 1/2 = 15 hours at 4°C, 10 hours at 21°C, and 6 hours at 40°C). During the first 6 hours after PN admixture compounding, an additive ascorbic acid loss of 4.6 ± 0.5 mg/L/ppm O 2 occurred because of residual O 2 in the bag. Conclusions: The new combination of plastic layers and careful O 2 monitoring during the filling process allowed near to complete prevention of ascorbic acid degradation in multilayered PN bags during 48 hours, regardless of the storage temperature.