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Effects of multilayered bags vs ethylvinyl‐acetate bags on oxidation of parenteral nutrition
Author(s) -
Balet A,
Cardona D,
Jane S,
MolinsPujol AM,
Sanchez Quesada JL,
Gich I,
Mangues MA
Publication year - 2004
Publication title -
journal of parenteral and enteral nutrition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.935
H-Index - 98
eISSN - 1941-2444
pISSN - 0148-6071
DOI - 10.1177/014860710402800285
Subject(s) - tbars , chemistry , dehydroascorbic acid , ascorbic acid , parenteral nutrition , chromatography , high performance liquid chromatography , lipid oxidation , thiobarbituric acid , antioxidant , food science , lipid peroxidation , surgery , medicine , biochemistry
BACKGROUND: We evaluate the effects of multilayered bags vs ethylvinyl‐acetate bags on peroxidate formation of various emulsions for all‐in‐one total parenteral nutrition solutions (TPN) during storage. METHODS: Twenty‐four parenteral nutritions were prepared with 4 commercial i.v. lipid emulsions (Soyacal 20%, Grifols; Intralipid 20%, Fresenius‐Kabi; Lipofundina 20%, Braun; and Clinoleic 20%, Clintex) and 2 different bags (multilayered [ML] bag, Miramed; and 1 ethylvinyl‐acetate [EVA] bag, Miramed). Each kind of TPN was prepared in triplicate. Samples were taken at 3 different times: immediately after preparation (time 0), after 6 days at 4 degrees C and 48 hours at 37 degrees C (time 1), and finally after a total of 14 days at 37 degrees C (time 2). Oxidation of TPN was evaluated by analysis of hydroperoxides by ferrous oxidation‐xylenol orange (FOX) reactive, lipoperoxides by thiobarbituric acid reactive species (TBARS), alpha‐tocopherol by high‐performance liquid chromatography (HPLC), and ascorbic acid and dehydroascorbic acid by HPLC. RESULTS: TPN admixtures in ML bag showed less oxidation evaluated by peroxide determination using FOX than EVA bag. Lipoperoxides by TBARS did not show significant differences between 2 bags. Ascorbic acid and dehydroascorbic acid disappeared in EVA bags at time 1. No important differences were found in alpha‐tocopherol content. CONCLUSIONS: Multilayered bags minimize oxidation.