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Effect of Tumor Weight and Tube Feeding on TNF‐α and IL‐1β mRNA Expression in the Brain of Mice
Author(s) -
Ozaki Kunihiro,
Yoshida Shogo,
Ishibashi Nobuya,
Kamei Hideki,
Muraoka Tatsuya,
Shirouzu Kazuo
Publication year - 2001
Publication title -
journal of parenteral and enteral nutrition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.935
H-Index - 98
eISSN - 1941-2444
pISSN - 0148-6071
DOI - 10.1177/0148607101025006317
Subject(s) - tumor necrosis factor alpha , cytokine , endocrinology , messenger rna , anorectic , medicine , biology , body weight , gene , biochemistry
Background: Previous studies have shown that cytokine mRNA expression is elevated in the brains of anorectic, tumor‐bearing rats. The objectives of the current study were as follows: (1) to determine whether a small tumor would result in up‐regulation of tumor necrosis factor (TNF)‐α and interleukin (IL)‐1β mRNA expression in the brain and other tissues of tumor‐bearing mice; and (2) to determine whether hyperalimentation by tube feeding would prevent up‐regulation of cytokine mRNA expression in the brain and other tissues of tumor‐bearing mice. Methods: Male C57BL/6 mice were divided into 4 groups: (1) control mice fed ad libitum (Control); (2) tumor‐bearing mice fed ad libitum (TB); (3) control mice receiving tube feeding (CTF); and (4) tumor‐bearing mice receiving tube feeding (TBTF). Results: TNF‐α and IL‐1β mRNA expression was elevated in the brains of mice with a 1% body weight tumor, relative to the control and CTF groups, without any detectable changes in the other organs. On the other hand, TNF‐α and IL‐1β mRNA expression was elevated in all organs of mice with an 8% body weight tumor, relative to the control and CTF groups. Tube feeding did not change TNF‐α and IL‐1β mRNA expression in mice burdened with either a 1% or 8% body weight tumor. Conclusions: Up‐regulation of cytokine mRNA synthesis occurs earlier in the brain than in other organs, and hyperalimentation does not change cytokine mRNA expression in tumor‐bearing mice. (Journal of Parenteral and Enteral Nutrition 25:317–322, 2001)