Premium
Reproducibility of Dorsal Hand Vein Responses to Phenylephrine and Prostaglandin F 2α Using the Dorsal Hand Vein Compliance Method
Author(s) -
Schindler C.,
Grossmann M.,
Dobrev D.,
Francke K.,
Ravens U.,
Kirch W.
Publication year - 2003
Publication title -
the journal of clinical pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.92
H-Index - 116
eISSN - 1552-4604
pISSN - 0091-2700
DOI - 10.1177/0091270002251004
Subject(s) - phenylephrine , medicine , vein , constriction , anesthesia , basal (medicine) , anatomy , blood pressure , insulin
Assessment of drug‐induced venodilation by the dorsal hand vein compliance method requires stable constriction of the vein. This study was designed to investigate intra‐ and intersubject reproducibility of the venous preconstriction technique in response to phenylephrine and prostaglandin F 2α and to determine the influence of basal vein size. Twelve healthy male nonsmokers participated in a prospective crossover study. Inter‐ and intrasubject variability was tested in response to phenylephrine and PGF 2α on different study days in the same hand vein. The dose of the respective constrictor causing approximately 80% constriction of the vein (ED 80 ) was determined and infused for another 100 minutes. Actual vein size was measured every 5 minutes. Coefficient of variation and regression analyses were performed to analyze influence of vessel size on ED 80 of the respective constrictor. Adjusted constriction levels were stable and well reproducible in all subjects. The intersubject coefficient of variation of ED 80 ranged from 0.9% to 6.7% for phenylephrine and from 0.9% to 6.9% for PGF 2α . Whereas responses to phenylephrine were independent of basal vein diameter, there was a positive correlation between ED 80 of PGF 2α and basal vein size. Thus, the hand vein compliance method is a suitable method to study dilatory responses in phenylephrine‐ or PGF 2α ‐constricted veins with considerable interindividual but small intraindividual variability. However, in such studies, phenylephrine appears to be a more reliable tool than PGF 2α .