Open AccessEarly Experience with a Highly Sensitive Bioassay for A.C.T.H.
Author(s) -
J. R. Daly,
N. Loveridge,
Lucille Bitensky,
J. Chayen
Publication year - 1972
Publication title -
annals of clinical biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.6
H-Index - 80
eISSN - 1758-1001
pISSN - 0004-5632
DOI - 10.1177/000456327200900141
Subject(s) - bioassay , zona reticularis , organ culture , guinea pig , staining , microbiology and biotechnology , chemistry , chromatography , in vitro , endocrinology , biology , medicine , pathology , biochemistry , zona glomerulosa , receptor , genetics , angiotensin ii
A new bioassay for A.C.T.H. has been devised utilising non-proliferative organ culture of segments of guinea pig adrenal. The cultured adrenal is exposed to standard or unknown A.C.T.H. and sections stained for the presence of reducing groups. The intensity of staining in the zona reticularis is measured by scanning and integrating microdensitometry and is inversely proportional to the logarithm of the concentration of A.C.T.H. in the culture medium over the range 0.0027–27 pg/ml. Preliminary results using the assay, which greatly exceeds others in sensitivity, on human plasma are similar to those obtained with standard techniques.