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Differentiation Patterns of Immortalized Human Meibomian Gland Epithelial Cells in Three-Dimensional Culture
Author(s) -
Nagayoshi Asano,
Ulrike Hampel,
Fabian Garreis,
Antje Schröder,
Martin Schicht,
Christian M. Hammer,
Friedrich Paulsen
Publication year - 2018
Publication title -
investigative ophthalmology and visual science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.935
H-Index - 218
eISSN - 1552-5783
pISSN - 0146-0404
DOI - 10.1167/iovs.17-23266
Subject(s) - meibomian gland , cell culture , keratin , keratinocyte , microbiology and biotechnology , chemistry , staining , pathology , biology , anatomy , medicine , surgery , genetics , eyelid
To establish a simplified three-dimensional (3D) meibomian gland culture model using a meibomian gland epithelial cell (HMGEC) line that might be a useful tool to gain deeper insights into meibomian gland dysfunction. For this purpose, 3D differentiation patterns and growth characteristics of HMGECs were studied on various membranes/scaffolds as well as in hanging drops.

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