Open AccessCutting and Decellularization of Multiple Corneal Stromal Lamellae for the Bioengineering of Endothelial Grafts
Author(s) -
Zhiguo Hé,
Fabien Forest,
Adeline Bernard,
AnneSophie Gauthier,
R. Montard,
Michel Péoc’h,
Clotilde Jumelle,
Emilie Courrier,
Chantal Perrache,
Philippe Gain,
Gilles Thuret
Publication year - 2016
Publication title -
investigative ophthalmology and visual science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.935
H-Index - 218
eISSN - 1552-5783
pISSN - 0146-0404
DOI - 10.1167/iovs.16-20256
Subject(s) - decellularization , stromal cell , cornea , biomedical engineering , tissue engineering , materials science , endothelial stem cell , ophthalmology , surgery , medicine , pathology , chemistry , in vitro , biochemistry
Engineered corneal endothelial grafts able to provide numerous functional endothelial cells for the restoration of corneal transparency would be a worthwhile way of replacing donor tissue, which is extremely scarce. The grafts are simply constructed: a biocompatible thin and transparent carrier colonized by a monolayer of cultured endothelial cells (ECs). Here we describe a process able to obtain appropriate carriers by recycling human corneas unsuitable for graft in their original state, but liable to provide multiple thin lamellae when cut with a femtosecond laser as used in refractive surgery.