Sleeping BeautyTransposon-Mediated Transfection of Retinal and Iris Pigment Epithelial Cells | Zendy

Sandra Johnen | Zendy; Zsuzsanna Izsvák | Zendy; Michael Stöcker | Zendy; Nina Harmening | Zendy; Anna Katharina Salz | Zendy; Peter Walter | Zendy; Gabriele Thumann | Zendy

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Sleeping BeautyTransposon-Mediated Transfection of Retinal and Iris Pigment Epithelial Cells

Author(s) -

Sandra Johnen,

Zsuzsanna Izsvák,

Michael Stöcker,

Nina Harmening,

Anna Katharina Salz,

Peter Walter,

Gabriele Thumann

Publication year - 2012

Publication title -

investigative ophthalmology and visual science

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.935

H-Index - 218

eISSN - 1552-5783

pISSN - 0146-0404

DOI - 10.1167/iovs.12-9951

Subject(s) - pedf , transfection , biology , microbiology and biotechnology , sleeping beauty transposon system , genetic enhancement , retinal , gene delivery , neuroprotection , immunology , cancer research , angiogenesis , gene , transposable element , genetics , pharmacology , biochemistry , genome

Subretinal transplantation of retinal (RPE) or iris (IPE) pigment epithelial cells has been advocated as a treatment for retinal degeneration. However, to our knowledge, in patients with age-related macular degeneration no significant beneficial effects on vision have been shown. Since the transplanted cells did not appear to maintain a healthy avascular and neuroprotective environment, we postulate that it will be necessary to transplant cells that express elevated levels of anti-angiogenic and neuroprotective activities. In our study, we provide a protocol for the efficient stable gene transfer and sustained gene expression of pigment epithelium-derived factor (PEDF), a potent anti-angiogenic and neuroprotective factor, using the nonviral Sleeping Beauty transposon system (SB100X).

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