Open AccessThe Kinome of Human Alveolar Type II and Basal Cells, and Its Reprogramming in Lung Cancer
Author(s) -
Sonia M. Leach,
Jay Finigan,
Vihas T. Vasu,
Rangnath Mishra,
Moumita Ghosh,
Daniel G. Foster,
Robert J. Mason,
Beata Kośmider,
Eveline Farias Hesson,
Jeffrey A. Kern
Publication year - 2019
Publication title -
american journal of respiratory cell and molecular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.469
H-Index - 161
eISSN - 1535-4989
pISSN - 1044-1549
DOI - 10.1165/rcmb.2018-0283oc
Subject(s) - kinome , reprogramming , biology , cancer research , lung cancer , kinase , microbiology and biotechnology , progenitor cell , cell , pathology , stem cell , medicine , genetics
The discovery of mutant tyrosine kinases as oncogenic drivers of lung adenocarcinomas has changed the basic understanding of lung cancer development and therapy. Yet, expressed kinases (kinome) in lung cancer progenitor cells, as well as whether kinase expression and the overall kinome changes or is reprogrammed upon transformation, is incompletely understood. We hypothesized that the kinome differs between lung cancer progenitor cells, alveolar type II cells (ATII), and basal cells (BC) and that their respective kinomes undergo distinct lineage-specific reprogramming to adenocarcinomas and squamous cell carcinomas upon transformation. We performed RNA sequencing on freshly isolated human ATII, BC, and lung cancer cell lines to define the kinome in nontransformed cells and transformed cells. Our studies identified a unique kinome for ATII and BC and changes in their kinome upon transformation to their respective carcinomas.