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Background   LIM  domain only 2 ( LMO 2, human gene) is a key transcription factor that regulates hematopoiesis and vascular development. However, its role in adult endothelial function has been incompletely characterized.    Methods and Results  In vitro loss‐ and gain‐of‐function studies on  LMO 2 were performed in human umbilical vein endothelial cells with lentiviral overexpression or short hairpin RNA knockdown ( KD ) of  LMO 2, respectively.  LMO 2  KD  significantly impaired endothelial proliferation.  LMO 2 controls endothelial G1/S transition through transcriptional regulation of cyclin‐dependent kinase 2 and 4 as determined by reverse transcription polymerase chain reaction (PCR), western blot, and chromatin immunoprecipitation, and also influences the expression of Cyclin D1 and Cyclin A1.  LMO 2  KD  also impaired angiogenesis by reducing transforming growth factor‐β (TGF‐β) expression, whereas supplementation of exogenous  TGF‐ β restored defective network formation in  LMO 2  KD  human umbilical vein endothelial cells. In a zebrafish model of caudal fin regeneration,  RT ‐ PCR  revealed that the lmo2 (zebrafish gene) gene was upregulated at day 5 postresection. The  KD  of lmo2 by vivo‐morpholino injections in adult  Tg ( fli1:egfp)   y1   zebrafish reduced 5‐bromo‐2′‐deoxyuridine incorporation in endothelial cells, impaired neoangiogenesis in the resected caudal fin, and substantially delayed fin regeneration.    Conclusions  The transcriptional factor  LMO 2 regulates endothelial proliferation and angiogenesis in vitro. Furthermore,  LMO 2 is required for angiogenesis and tissue healing in vivo. Thus,  LMO 2 is a critical determinant of vascular and tissue regeneration.

LIM Domain Only 2 Regulates Endothelial Proliferation, Angiogenesis, and Tissue Regeneration