Open AccessLIM Domain Only 2 Regulates Endothelial Proliferation, Angiogenesis, and Tissue Regeneration
Author(s) -
Meng Shu,
Matrone Gianfranco,
Lv Jie,
Chen Kaifu,
Wong Wing Tak,
Cooke John P.
Publication year - 2016
Publication title -
journal of the american heart association
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.494
H-Index - 85
ISSN - 2047-9980
DOI - 10.1161/jaha.116.004117
Subject(s) - angiogenesis , zebrafish , biology , microbiology and biotechnology , gene knockdown , vascular endothelial growth factor b , vascular endothelial growth factor a , vascular endothelial growth factor , cancer research , cell culture , biochemistry , genetics , vegf receptors , gene
Background LIM domain only 2 ( LMO 2, human gene) is a key transcription factor that regulates hematopoiesis and vascular development. However, its role in adult endothelial function has been incompletely characterized. Methods and Results In vitro loss‐ and gain‐of‐function studies on LMO 2 were performed in human umbilical vein endothelial cells with lentiviral overexpression or short hairpin RNA knockdown ( KD ) of LMO 2, respectively. LMO 2 KD significantly impaired endothelial proliferation. LMO 2 controls endothelial G1/S transition through transcriptional regulation of cyclin‐dependent kinase 2 and 4 as determined by reverse transcription polymerase chain reaction (PCR), western blot, and chromatin immunoprecipitation, and also influences the expression of Cyclin D1 and Cyclin A1. LMO 2 KD also impaired angiogenesis by reducing transforming growth factor‐β (TGF‐β) expression, whereas supplementation of exogenous TGF‐ β restored defective network formation in LMO 2 KD human umbilical vein endothelial cells. In a zebrafish model of caudal fin regeneration, RT ‐ PCR revealed that the lmo2 (zebrafish gene) gene was upregulated at day 5 postresection. The KD of lmo2 by vivo‐morpholino injections in adult Tg ( fli1:egfp) y1 zebrafish reduced 5‐bromo‐2′‐deoxyuridine incorporation in endothelial cells, impaired neoangiogenesis in the resected caudal fin, and substantially delayed fin regeneration. Conclusions The transcriptional factor LMO 2 regulates endothelial proliferation and angiogenesis in vitro. Furthermore, LMO 2 is required for angiogenesis and tissue healing in vivo. Thus, LMO 2 is a critical determinant of vascular and tissue regeneration.