Open AccessTransglutaminase is a Critical Link Between Inflammation and Hypertension
Author(s) -
Luo Renna,
Liu Chen,
Elliott Serra E.,
Wang Wei,
Parchim Nicholas,
Iriyama Takayuki,
Daugherty Patrick S.,
Tao Lijian,
Eltzschig Holger K.,
Blackwell Sean C.,
Sibai Baha M.,
Kellems Rodney E.,
Xia Yang
Publication year - 2016
Publication title -
journal of the american heart association
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.494
H-Index - 85
ISSN - 2047-9980
DOI - 10.1161/jaha.116.003730
Subject(s) - medicine , tissue transglutaminase , endocrinology , pathogenesis , kidney , receptor , autoantibody , hypoxia (environmental) , proteinuria , inflammation , immunology , enzyme , antibody , biology , biochemistry , chemistry , organic chemistry , oxygen
Background The pathogenesis of essential hypertension is multifactorial with different underlying mechanisms contributing to disease. We have recently shown that TNF superfamily member 14 LIGHT (an acronym for homologous to lymphotoxins, exhibits inducible expression, and competes with herpes simplex virus glycoprotein D for herpes virus entry mediator, a receptor expressed by T lymphocytes, also known as TNFSF 14) induces hypertension when injected into mice. Research reported here was undertaken to examine the role of transglutaminase ( TG ase) in LIGHT ‐induced hypertension. Methods and Results Initial experiments showed that plasma and kidney TG ase activity was induced by LIGHT infusion (13.91±2.92 versus 6.75±1.92 mU/mL and 19.86±3.55 versus 12.00±0.97 mU/10 μg) and was accompanied with hypertension (169±7.16 versus 117.17±11.57 mm Hg at day 14) and renal impairment (proteinuria, 61.33±23.21 versus 20.38±9.01 μg/mg; osmolality, 879.57±93.02 versus 1407.2±308.04 mmol/kg). The increase in renal TG ase activity corresponded to an increase in RNA for the tissue TGase isoform, termed TG 2. Pharmacologically, we showed that LIGHT ‐induced hypertension and renal impairment did not occur in the presence of cystamine, a well‐known competitive inhibitor of TG ase activity. Genetically, we showed that LIGHT ‐mediated induction of TG ase, along with hypertension and renal impairment, was dependent on interleukin‐6 and endothelial hypoxia inducible factor‐1α. We also demonstrated that interleukin‐6, endothelial hypoxia inducible factor‐1α, and TG ase are required for LIGHT ‐induced production of angiotensin receptor agonistic autoantibodies. Conclusions Thus, LIGHT ‐induced hypertension, renal impairment, and production of angiotensin receptor agonistic autoantibodies require TG ase, most likely the TG 2 isoform. Our findings establish TG ase as a critical link between inflammation, hypertension, and autoimmunity.