Open AccessMembrane‐Tethered Metalloproteinase Expressed by Vascular Smooth Muscle Cells Limits the Progression of Proliferative Atherosclerotic Lesions
Author(s) -
Barnes Richard H.,
Akama Takeshi,
Öhman Miina K.,
Woo MoonSook,
Bahr Julian,
Weiss Stephen J.,
Eitzman Daniel T.,
Chun TaeHwa
Publication year - 2017
Publication title -
journal of the american heart association
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.494
H-Index - 85
ISSN - 2047-9980
DOI - 10.1161/jaha.116.003693
Subject(s) - vascular smooth muscle , apolipoprotein e , matrix metalloproteinase , knockout mouse , extracellular matrix , biology , microbiology and biotechnology , endocrinology , pathology , medicine , smooth muscle , gene , biochemistry , disease
Background The MMP (matrix metalloproteinase) family plays diverse and critical roles in directing vascular wall remodeling in atherosclerosis. Unlike secreted‐type MMP s, a member of the membrane‐type MMP family, MT 1‐ MMP (membrane‐type 1 MMP; MMP 14), mediates pericellular extracellular matrix degradation that is indispensable for maintaining physiological extracellular matrix homeostasis. However, given the premature mortality exhibited by MT 1‐ MMP –null mice, the potential role of the proteinase in atherogenesis remains elusive. We sought to determine the effects of both MT 1‐ MMP heterozygosity and tissue‐specific gene targeting on atherogenesis in APOE (apolipoprotein E)–null mice. Methods and Results MT 1‐ MMP heterozygosity in the APOE ‐null background ( Mmp14 +/− Apoe −/− ) significantly promoted atherogenesis relative to Mmp14 +/+ Apoe −/− mice. Furthermore, the tissue‐specific deletion of MT 1‐ MMP from vascular smooth muscle cells ( VSMC s) in SM 22α‐Cre(+) Mmp14 F/F Apoe −/− ( VSMC ‐knockout) mice likewise increased the severity of atherosclerotic lesions. Although VSMC ‐knockout mice also developed progressive atherosclerotic aneurysms in their iliac arteries, macrophage‐ and adipose‐specific MT 1‐ MMP –knockout mice did not display this sensitized phenotype. In VSMC ‐knockout mice, atherosclerotic lesions were populated by hyperproliferating VSMC s (smooth muscle actin– and Ki67–double‐positive cells) that were characterized by a proinflammatory gene expression profile. Finally, MT 1‐ MMP –null VSMC s cultured in a 3‐dimensional spheroid model system designed to mimic in vivo–like cell–cell and cell–extracellular matrix interactions, likewise displayed markedly increased proliferative potential. Conclusions MT 1‐ MMP expressed by VSMC s plays a key role in limiting the progression of atherosclerosis in APOE ‐null mice by regulating proliferative responses and inhibiting the deterioration of VSMC function in atherogenic vascular walls.