Open AccessCirculating microRNA Profiling Needs Further Refinement Before Clinical Use in Patients With Aortic Stenosis
Author(s) -
Coffey Sean,
Williams Michael J. A.,
Phillips L. Vicky,
Jones Gregory T.
Publication year - 2015
Publication title -
journal of the american heart association
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.494
H-Index - 85
ISSN - 2047-9980
DOI - 10.1161/jaha.115.002150
Subject(s) - medicine , coronary artery disease , stenosis , cardiology , biomarker , cohort , microrna , disease , gene , biochemistry , chemistry
Background Aortic stenosis (AS) is a progressive condition leading to heart failure and death without treatment. No medical therapy currently exists for AS, and a major management challenge is deciding on the correct timing of aortic valve replacement. MicroRNAs (mi RNA s) are short noncoding RNA s that are stable in the circulation. We wished to use mi RNA s as biomarkers of disease in AS . Methods and Results We performed microarray‐based whole mi RN ome profiling of 24 participants with AS and 27 control participants. After adjustment for age and multiple testing, we identified 4 mi RNA s significantly different between groups. These findings were then examined using quantitative polymerase chain reaction in a larger validation cohort of 101 controls and 94 participants with AS, stratified in a prespecified analysis by presence of coexisting coronary artery disease ( CAD ). We obtained mixed results for miR‐22‐3p, miR‐24‐3p, miR‐382‐5p, and miR‐451a in the validation cohort, with differing associations according to CAD status. miR‐21‐5p was increased in AS patients without CAD, but there was no difference between groups with CAD . Conclusion Despite holding great promise, circulating mi RNA profiling requires further refinement before translation into clinical use as a biomarker in aortic stenosis.