Open AccessCD84 Links T Cell and Platelet Activity in Cerebral Thrombo-Inflammation in Acute Stroke
Author(s) -
Michael K. Schuhmann,
Guido Stoll,
Michael Bieber,
Timo Vögtle,
Sebastian Hofmann,
Vanessa Klaus,
Peter Kraft,
Mert Seyhan,
Alexander M. Kollikowski,
Lena Papp,
Peter U. Heuschmann,
Mirko Pham,
Bernhard Nieswandt,
David Stegner
Publication year - 2020
Publication title -
circulation research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 4.899
H-Index - 336
eISSN - 1524-4571
pISSN - 0009-7330
DOI - 10.1161/circresaha.120.316655
Subject(s) - platelet , stroke (engine) , medicine , inflammation , effector , platelet activation , immunology , infiltration (hvac) , cardiology , mechanical engineering , physics , engineering , thermodynamics
Rationale: Ischemic stroke is a leading cause of morbidity and mortality worldwide. Recanalization of the occluded vessel is essential but not sufficient to guarantee brain salvage. Experimental and clinical data suggest that infarcts often develop further due to a thromboinflammatory process critically involving platelets and T cells, but the underlying mechanisms are unknown. Objective: We aimed to determine the role of CD (cluster of differentiation)-84 in acute ischemic stroke after recanalization and to dissect the underlying molecular thromboinflammatory mechanisms. Methods and Results: Here, we show that mice lacking CD84—a homophilic immunoreceptor of the SLAM (signaling lymphocyte activation molecule) family—on either platelets or T cells displayed reduced cerebral CD4+ T-cell infiltration and thrombotic activity following experimental stroke resulting in reduced neurological damage. In vitro, platelet-derived soluble CD84 enhanced motility of wild-type but not ofCd84 −/− CD4+ T cells suggesting homophilic CD84 interactions to drive this process. Clinically, human arterial blood directly sampled from the ischemic cerebral circulation indicated local shedding of platelet CD84. Moreover, high platelet CD84 expression levels were associated with poor outcome in patients with stroke.Conclusions: These results establish CD84 as a critical pathogenic effector and thus a potential pharmacological target in ischemic stroke.