Open AccessMeasurement of Myofilament-Localized Calcium Dynamics in Adult Cardiomyocytes and the Effect of Hypertrophic Cardiomyopathy Mutations
Author(s) -
Alexander J. Sparrow,
Kolja Sievert,
Suketu Patel,
YuanShiun Chang,
Connor N Broyles,
Frances A. Brook,
Hugh Watkins,
Michael A. Geeves,
Charles Redwood,
Paul Robinson,
Matthew J. Daniels
Publication year - 2019
Publication title -
circulation research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 4.899
H-Index - 336
eISSN - 1524-4571
pISSN - 0009-7330
DOI - 10.1161/circresaha.118.314600
Subject(s) - myofilament , sarcomere , troponin i , medicine , troponin , actin , troponin c , troponin complex , cardiomyopathy , cardiology , myofibril , hypertrophic cardiomyopathy , troponin t , levosimendan , myocyte , heart failure , biophysics , chemistry , biology , microbiology and biotechnology , myocardial infarction
Subcellular Ca 2+ indicators have yet to be developed for the myofilament where disease mutation or small molecules may alter contractility through myofilament Ca 2+ sensitivity. Here, we develop and characterize genetically encoded Ca 2+ indicators restricted to the myofilament to directly visualize Ca 2+ changes in the sarcomere.