Open AccessTumor necrosis factor alpha -308 promoter polymorphism and insulin resistance inoverweight and obese adolescent
Author(s) -
Carlos Alberto Menezes,
Luís Jesuino de Oliveira Andrade,
Jordian Jorge Pinheiro,
Gustavo Soares Correia,
Paulo Roberto Santana de Melo,
Fabrício Rios-Santos
Publication year - 2018
Publication title -
medicina
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.104
H-Index - 10
eISSN - 2176-7262
pISSN - 0076-6046
DOI - 10.11606/issn.2176-7262.v50i6p358-364
Subject(s) - insulin resistance , genotype , overweight , allele , medicine , obesity , promoter , endocrinology , polymorphism (computer science) , biology , genetics , gene , gene expression
Study Model/Methodology: This is a cross-sectional study with a sample of 104 overweight/obese adolescents, with a mean weight of 52.98 kg ± 22.00, mean age 16.01 ± 2.91 years. We used the homeostasis model assessment-estimated IR (HOMA-IR) index to quantify the insulin resistance (IR). The -308 polymorphism of the promoter of TNF-α was performed using polymerase chain reactionrestriction fragment length polymorphism technique. Statistical analysis of the quantitative measures was conducted with a student’s t-test. For correlation between the genotype and alleles, we used chisquare statistical test. To test the heterogeneity between HOMA-IR and the anthropometric parameters the Mann-Whitney test was used, associated with the Hardy-Weinberg equilibrium. The association between -308G/A polymorphism of the promoter of TNF-α and HOMA-IR was tested by univariate linear regression analysis. Objective: Investigate the association between -308G/A polymorphism in the promoter of tumor necrosis factor-alpha (TNF-α) and susceptibility to IR in overweight/obese adolescents. Results: The prevalence of IR was 18.30% according to the HOMA-IR. The frequency of GG, AG and AA genotype was found 75 (72.12%), 28 (26.92%) and 1.0 (0.96%) respectively. Allele frequencies for guanine (G) and adenine (A) were 178 (85.58%) and 30 (14.42%), respectively. The allele A as well as GA and AA genotype contributed to increase RI (14.42% and 27.88% respectively). Conclusion: The - 308 G/A polymorphism of the promoter of TNF-α can contribute to the IR increase in obese adolescents with GA and AA genotypes.