Open AccessEffect of cryoprotectant on the motility, viability, fertilization, and DNA integrity of naleh fish Barbonymus sp. (Cyprinidae) sperm
Author(s) -
Siti Maulida,
Kartini Eriani,
Firman M. Nur,
Nur Fadli,
Agung Setia Batubara,
Abdullah A. Muhammadar,
Siti Azizah Mohd Nor,
Martin Wilkes,
Zainal A. Muchlisin
Publication year - 2021
Publication title -
brazilian journal of veterinary research and animal science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.187
H-Index - 17
eISSN - 1678-4456
pISSN - 1413-9596
DOI - 10.11606/issn.1678-4456.bjvras.2021.168702
Subject(s) - cryoprotectant , cryopreservation , sperm , biology , human fertilization , yolk , extender , dna fragmentation , sperm motility , population , andrology , glycerol , semen , zoology , chemistry , fishery , botany , anatomy , biochemistry , embryo , medicine , apoptosis , demography , organic chemistry , programmed cell death , sociology , polyurethane
Naleh fish Barbonymus sp. is a commercial freshwater fish, which is indigenous to Aceh, Indonesia. The population of this species has declined over the years as a result of habitat perturbations and overfishing. Hence, the crucial need to develop a cryopreservation method to support breeding programs. This involved the use of a cryoprotectant as an important component. The objective of this study, therefore, was to explore the best cryoprotectant for naleh fish spermatozoa, and a total of five types were tested. These include the DMSO, Methanol, Ethanol, Glycerol, and Ethylene Glycol at a similar concentration of 10%, which were individually combined with 15% egg yolk, and every treatment was performed in three replications. Conversely, Ringer’s solution was adopted as an extender, and the sperm was cryopreserved in liquid nitrogen for 15 days. The results showed significant influence on sperm motility and viability, as well as egg fertility of naleh fish (P <0.05), although the DMSO provided the best outcome, compared to others at 47.17%, 50.13%, and 45.67%, respectively. Furthermore, DNA fragmentation had not occurred in the fresh and cryopreserved sperm samples, indicating the protective effect of tested cryoprotectants. It is concluded that the 10% DMSO and 15% egg yolk is the best cryoprotectant for naleh fish spermatozoa.