Open AccessEffect of single dose follicle stimulating hormone on follicular aspiration, in vitro fertilization and pregnancy rate
Author(s) -
Mayara Ellen Bardi de Moraes,
Paulo Roberto Adona,
Samuel Guemra,
Tiago Henrique Camara De,
M. S. Miranda
Publication year - 2019
Publication title -
brazilian journal of veterinary research and animal science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.187
H-Index - 17
eISSN - 1678-4456
pISSN - 1413-9596
DOI - 10.11606/issn.1678-4456.bjvras.2019.156894
Subject(s) - follicular phase , in vitro fertilisation , human fertilization , pregnancy , follicle , andrology , pregnancy rate , follicle stimulating hormone , oocyte , medicine , hormone , biology , embryo , endocrinology , luteinizing hormone , anatomy , microbiology and biotechnology , genetics
The present study evaluated Brangus cows treated with single doses of follicle stimulating hormone (FSH) subjected to follicular aspiration after 24 h to assess oocyte recovery, in vitro fertilization and pregnancy rate. Follicles exceeding 3 millimeters in diameter were aspirated, 200 mg of FSH was administered 2 days later, and a new ovum pickup was performed 24 h afterward. These methods were performed 3 times every 3 days. In control, follicular aspirations occurred at intervals of 1-week without FSH administration o. The aspirated oocytes were evaluated, submitted to in vitro fertilization and the embryos were transferred to the recipients. The average recovery of oocytes was higher (p 0.05) in the mean percentage of viable oocytes (52.0±3.9 and 62.7±4.7%) or the mean percentage of embryos (41.4±4.8 and 41.5±4.2%) among control and treated cows, respectively. The mean percentage of pregnancy did not differ (p>0.05) for control cows (43.8±2.7%), and treated cows (40.9±6.8%). In conclusion, FSH treatment did not improve oocyte recovery, in vitro fertilization, and pregnancy percentage. However, there is possibility of several consecutive ovum pickup every t3 days, concentrating the in vitro fertilization and the pregnancy percentage.