Open AccessHuman Papillomavirus types distribution among women with cervical preneoplastic, lesions and cancer in Luanda, Angola
Author(s) -
Paciência de Almeida Damião,
Michelle Oliveira-Silva,
Miguel Ângelo Martins Moreira,
Natalia Poliakova,
Maria Emilia Rt de Lima,
José Chiovo,
Alcina F. Nicol
Publication year - 2016
Publication title -
the pan african medical journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.287
H-Index - 30
ISSN - 1937-8688
DOI - 10.11604/pamj.2016.24.268.9678
Subject(s) - ascus (bryozoa) , medicine , cervical cancer , cancer , human papillomavirus , gynecology , hpv infection , squamous intraepithelial lesion , oncology , cervical intraepithelial neoplasia , biology , microbiology and biotechnology , ascospore , spore
Cervical cancer is the leading cause of cancer deaths among females in Angola and human papillomavirus (HPV) is the main risk factor for the development of pre-cancerous squamous intraepithelial lesions. The diversity and frequency of HPV types in Angola has yet to be reported. Aim To determine the frequency of HPV among women with squamous intraepithelial lesions from women in Luanda, Angola. Methods Study participants included women diagnosed with cytological abnormalities that voluntarily provided Pap smears (n = 64). Genomic DNA was extracted from the samples for use as templates in the PCR amplification of HPV sequences. PCR products were sequenced to determine HPV type. Results HPV DNA was detected in 71.9% (46/64) in the samples. A higher diversity of HPV types was found in the cytological lesions, such as ASCUS and LSIL (HPV16, 6, 18, 31, 58, 66, 70 and 82, in order of frequency) than that detected for HSIL and SSC (HPV16, 18, 6 and 33). The most prevalent HPV type were: HPV16, HPV6 and HPV18. Conclusion This is the first report on HPV type diversity and frequency in woman of Angola. The results suggest that large-scale studies across Africa would improve our understanding of interrelationship between HPV infections and cervical cancer. More directly, the identification of the HPV types most prevalent suggests that women in Angola would benefit from currently available HPV vaccines.