Open AccessПероксидне окиснення білків стінки тонкої кишки, міокарда та печінки щурів при експериментальному застосуванні карагінану
Author(s) -
М. І. Марущак,
O. M. Kopanitsa,
Іnna Krynytska,
Т. Ya. Yaroshenko
Publication year - 2018
Publication title -
medična ì klìnìčna himiâ
Language(s) - English
Resource type - Journals
eISSN - 2414-9934
pISSN - 2410-681X
DOI - 10.11603/mcch.2410-681x.2017.v0.i4.8435
Subject(s) - carrageenan , chemistry , small intestine , gastrointestinal tract , biochemistry , medicine , food science
. In the last decades, the carrageenans have become one of the most popular hydrocolloids in the food industry. In addition to the positive properties of hydrocolloids, researchers have established the relationship between the incidence of ulcerative colitis and the level of carrageenan consumption, which makes it considered as a potential etiological factor in the pathology of the gastrointestinal tract.The aim of the study – to determine the level of oxidative modification of proteins of the wall of the small intestine, tissues of the heart and liver of rats using 1% κ-carrageenan solution.Materials and Methods. The study was carried out on 24 white non-linear male rats. The animals of the experimental group were provided with free access to a 1.0% of carrageenan solution for 1 month. In the selected samples of the small intestine, heart and liver was evaluated the peroxidic oxidation of proteins according to the level of their oxidation modifications products using spectrophotometry at wavelengths of 370 and 430 nm.Research Results. A significant increasing of ketone dinitrophenylhydrazones levels in the small intestine wall (by 57.0%) and in the liver (by 23.0%) and, respectively, the aldehyde dinitrophenylhydrazones levels by 47.7% and 19.1%, compared with the control values (p<0.01). In this case, the peroxide oxidation, to a greater extent, was subjected to neutral proteins. It should be noted that the peroxidic oxidation of proteins in the myocardium of the rats with carrageenan consumption, recorded at a wavelength of 370 nm, exceeded the control data by 14.3% (p<0.05).Conclusions. Oral administration of a 1% carrageenan solution in experimental animals leads to statistically significant activation statistically significant activation of peroxidation of proteins in the wall of the small intestine and the liver, whereas only the growth of neutral ketone dinitrophenylhydrazones is registered in the myocardium (p<0.05).