Open AccessOptimization of human somatic cells cryopreservation protocols by polyethylene glycols
Author(s) -
Olena Perepelytsina,
A. P. Ugnivenko,
D. P. Burlaka,
S. V. Bezugly,
M. V. Sydorenko
Publication year - 2016
Publication title -
medična ì klìnìčna himiâ
Language(s) - English
Resource type - Journals
eISSN - 2414-9934
pISSN - 2410-681X
DOI - 10.11603/mcch.2410-681x.2016.v0.i3.6933
Subject(s) - vitrification , cryopreservation , somatic cell , polyethylene glycol , peg ratio , chemistry , andrology , biochemistry , microbiology and biotechnology , biology , embryo , medicine , finance , gene , economics
Using of polyethylene glycol in optimization of human somatic cells of cryopreservation protocols was analyzed. Low molecular weight PEG as vitrification solution supplement exhibited a high cooling speed and provided cell survival in 200 % comparing with control. It allows recommending the use of low molecular weight PEG in vitrification environment for effective cell vitrification protocols.