ДОСЛІДЖЕННЯ РАНОЗАГОЮВАЛЬНОЇ ДІЇ МАЗІ З ЕКСТРАКТОМ СОСНИ ЗВИЧАЙНОЇ

RESEARCH OF THE WOUND HEALING ACTION OF THE OINTMENT WITH PINE EXTRACT1T . P . Mandzii , 2 А. R . Grytsyk , 3 А. О. Kl y menko, 4 І. О. Nyektyehayev 1,2,3 SHEI "Ivano - Frankivsk national medical university", Ivano – Frankivsk 4 SHEI "Lviv national medical university named after Danylo Galyts’kiy", Lviv   Introduction . The range of medicines intended for the local conservative treatment of wounds is growing steadily. Currently, there is no universal drug suitable for application in all phases of wound healing process: purulent-necrotic, phases of regeneration (the formation of granulation tissue) and scarring, because each phase of treatment requires a differentiated selection of drugs with different mechanisms of action. Worked out literature sources have shown the prospect of pine dosage form development. Pinaceae family consists of 10 genera and around 250 species and is the most common group among coniferous class and among gymnosperms in general. Pinus genus includes the greatest number of species. It contains about 100 species represented by evergreen trees. The most widespread in Ukraine is pine ( Pinus s y lvestris L.). In medical practice pine buds ( Gemmae Pini , or Turiones pini ) and products derived from pine, such as turpentine ( Oleum Therebintyinae ), essential oil ( Oleum Pini sylvestris ), rosin ( Pix liquida Pini ) and activated charcoal ( Carbo activata ) are used. Investigationmethods . Wound healing activity was studied in experiments in vivo on 32 white outbred rats according to the "General ethical principles of animal experimentation" (Ukraine, 2001). Experiment was conducted on animals weighing 170 -192 g, who were on a general diet and kept in the same conditions. To reproduct the model of linear wound rats were introduced into barbamil anesthesia (0,8 ml of 1 % aqueous solution at barbami for 100 mg of body weight). After their hair was cut off, a piece of skin sized 1×1 cm was carved in aseptic conditions (operating area was moistened with 5% alcoholic solution of iodine). Results and discussion . The obtained results indicate that the acceleration of wound healing process was observed under the application of the ointment with extract of pine (reduction of wound area to 50,10 ± 3,34 and 2,13 ± 0,52 mm 2 on the 7 th and 14 th day respectively). Thus, ointment with extract of pine as the medicine for the treatment of wounds in the experiment prevails the referent medicine (group 4) and provides wound healing in a shorter time. Under visual observation of group number 2 the change in wound area on the 7 th and 14 th day (50,10 and 2,13 mm 2 respectively) was observed. The development of wound process was accompanied by changes in protein metabolism and level of middle mass molecules (total protein of animals of the control group and the group number 2 was  45,6 ± 1,17 g/l and 57,4 ± 2,9 g/l respectively, the content of middle mass molecules was 0,29 ± 0,02 standard units and 0,22 ± 0,01 standard units respectively). Conclusions .The results of conducted researches show that the application of an ointment with pine extract for the treatment of wound process provides wound healing at a shorter time. Reparative activity of the ointment with pine extract applied to the wound area shows more pronounced activity in comparison with the referent medicine. References1. Shalymov O. O. Suchasne medykamentozne likuvannya ran / O. O. Shalymov, V. F. Sayenko, B. M. Datsenko // Vidomcha instruktsiya.– K., 2002.– 37 s. 2. Martin P. Inflammatory cells during wound repair: the good, the bad and the ugly / P. Martin, S. J. Leibovich // Trends Cell Biol.– 2005.– V. 15, № 4.– R. 599–607. 3. Velnar T. The wound healing process: an overview of the cellular and molecular mechanisms / T. Velnar, T. Bailey, V. Smrkolj // J. Int. Med. Res.– 2009.– V. 37, № 5.– R. 1528–1542. 4. Zast·souvannya roslyn klasu khvoyni u medytsyni. Rodyna Sosnovi (Ohlyad literatury) – T. M. Kozymenko, L. H. Dudchenko, T. YU. Hrabova [ta inshi] - Fitoterapiya. Chasopys – №2 – K.: 2014 – S. 34 – 39. 5. Ranozazhyvlyayushchaya aktyvnostʹ mazy, soderzhashchey éfyrnoe maslo travy tysyachelystnyka poymennoho / S. D. Trzhetsynskyy, V. Y. Mozulʹ, H. A. Zhernov [y dr.] – Naukovo - praktychnyy zhurnal – Zaporizhzhya : Aktualʹni pytannya farmatsevtychnoyi i medychnoyi nauky ta praktyky, № 2 (15) – 2014. – S. 50 – 53. 6. Doklinichni doslidzhennya likarsʹkykh zasobiv: Metodychni rekomendatsiyi / Za red. chl.-kor. AMN Ukrayiny O. V. Stefanova. – K.: Avitsena, 2001. – 528 s. 7. Teoryya y praktyka mestnoho lechenyya hnoynykh ran (problemy lekarstvennoy terapyy) / Pod red. B. M. Datsenko. – K.: Zdorov'ya, 1995. – 383 s. 8. Rany y ranevaya ynfektsyya: Rukovodstvo dlya vrachey / Pod red. M. Y. Kuzyna, B. M. Kostyuchonok. – 2-e yzd. – M.: Medytsyna, 1990. – 592 s. 9. Kamyshnykov V. S. Spravochnyk po klynyko - byokhymycheskoy laboratornoy dyahnostyke: V 2 t. – Mn.: Belarusʹ, 2000. – T. 1. – 495 s.