Open AccessPotential and Performance of Accelerated Solvent Extraction (ASE) in Obtaining Bioactive Compounds from Bee Propolis: Comparison with Soaking, Ultrasonication, and Microwave-Assisted Methods
Author(s) -
Elmer-Rico E. Mojica,
Jozlyn M Charland,
Custer C. Deocaris,
Jose Rene L. Micor
Publication year - 2021
Publication title -
journal of tropical life science
Language(s) - English
Resource type - Journals
eISSN - 2527-4376
pISSN - 2087-5517
DOI - 10.11594/jtls.11.02.08
Subject(s) - propolis , extraction (chemistry) , maceration (sewage) , accelerated solvent extraction , sonication , chromatography , absorbance , solvent , chemistry , yield (engineering) , materials science , food science , organic chemistry , metallurgy , composite material
Propolis is a natural resinous substance collected by honeybees from buds and exudates of trees. The material has attracted much attention in recent years as a functional food component since it possesses various biological properties, including antimicrobial, antioxidative, and anti-ulcer properties. In this study, the performance of accelerated solvent extraction (ASE) was assessed and compared with varying methods of extraction: soaking (maceration), ultrasonication, and microwave-assisted methods. Gas chromatography-mass spectrometry (GC-MS) and other spectroscopic techniques, such as absorbance and fluorescence, were employed to assess the efficiency in the extraction of natural products. The antioxidant activity and phenolic content of the different extracts were also determined. Results showed samples obtained from the microwave method showed the highest yield in the extraction of bioactive compounds. Although microwave showed the best method in this study, some issues and recommendations on ASE application for extracting natural products from bee propolis were discussed. Given the ease in controlling extraction temperature with ASE, this technique has a great potential to be a better method for extraction of heat-labile natural products from propolis should optimization of conditions for extraction were further performed.