Open AccessPotency of Endophytic and Rhizospheric Bacteria of Akar Kucing (Acalypha indica Linn.) as Antibacteria against Klebsiella pneumoniae
Author(s) -
Fitriana Dewi Rahmawati,
Suharjono Suharjono
Publication year - 2021
Publication title -
journal of tropical life science
Language(s) - English
Resource type - Journals
eISSN - 2527-4376
pISSN - 2087-5517
DOI - 10.11594/jtls.11.02.06
Subject(s) - bacteria , biology , klebsiella pneumoniae , microbiology and biotechnology , klebsiella pneumonia , rhizosphere , pathogenic bacteria , klebsiella , antibacterial activity , staphylococcus aureus , escherichia coli , biochemistry , genetics , gene
The prevalence of pneumonia in Indonesia was 2% in 2018. Treatment of pneumonia using antibiotics caused resistance to pathogenic bacteria. Endophytic and rhizospheric bacteria from the medicinal plant Acalypha indica Linn., is a new type of bacteria that produces antibacterial compounds against Klebsiella pneumoniae. This study aims to analyze the potency and identify endophytic and rhizospheric bacteria of the A. indica Linn as an antibacterial of K. pneumoniae. The research includes isolation and purification of endophytic and rhizospheric bacteria of the A. indica Linn, antagonistic assay of bacteria cell culture, antibacterial assay of bacteria metabolites, and identification of potential isolates based on 16S rDNA sequence similarity. The results showed that number of rhizosphere bacteria 6.83 × 105 CFU/g was more than endophytic bacteria 1.78 × 104 CFU/g. Diversity of rhizosphere bacterial 0.72 was higher than endophytic bacteria 0.62. The rhizospheric bacteria RU112B and RU315B had the highest activity to inhibit the growth of K. pneumoniae. Both isolates RU112B and RU315B were identified as Staphylococcus saprophyticus with a similarity 99.83% and Luteimonas terrae with a similarity 99.67% respectively