Open AccessDisinfection Effect of Hexadecyl Pyridinium Chloride on SARS-CoV-2 in vitro
Author(s) -
Chen Ke-da,
Ma Fei-ke,
Wang Qing-jing,
Wang Ying,
Zhuang Xin-yi,
Zhang Xu-ning,
Mao Hai-yan,
Zhang Yan-jun
Publication year - 2022
Publication title -
intervirology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.641
H-Index - 61
eISSN - 1423-0100
pISSN - 0300-5526
DOI - 10.1159/000526241
Subject(s) - brief report
The novel coronavirus (COVID-19 or 2019-nCoV) is a respiratory virus that can exist in the mouth and saliva of patients and spreads through aerosol dispersion. Therefore, stomatological hospitals and departments have become high-infection-risk environments. Accordingly, oral disinfectants that can effectively inactivate the virus have become a highly active area of research. Hexadecyl pyridinium chloride, povidone-iodine, and other common oral disinfectants are the natural primary choices for stomatological hospitals. Therefore, this study investigated the inhibitory effect of hexadecyl pyridinium chloride on severe acute respiratory syndrome coronavirus (SARS-CoV-2) in vitro. Vero cells infected with SARS-CoV-2 were used to determine the disinfection effect; the CCK-8 method was used to determine cytotoxicity, and viral load was determined by real-time PCR. The results showed that hexadecyl pyridinium chloride has no obvious cytotoxic effect on Vero cells in the concentration range of 0.0125–0.05 mg/mL. The in vitro experiments showed that hexadecyl pyridinium chloride significantly inhibits the virus at concentrations of 0.1 mg/mL or above at 2 min of action. Thus, the results provide experimental support for the use of hexadecyl pyridinium chloride in stomatological hospitals.