Open AccessUrinary Podocyte Count as a Potential Routine Laboratory Test for Glomerular Disease: A Novel Method Using Liquid-Based Cytology and Immunoenzyme Staining
Author(s) -
Junichi Sakane,
Hirotsugu Kitayama,
Takashi Inoue,
Akihiro Nakamura,
Masayoshi Yamada,
Yudai Miyama,
Hideki Kawamura,
Hideto Iwafuchi,
Shingo Kamoshida,
Hiroyuki Ohsaki
Publication year - 2022
Publication title -
acta cytologica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.513
H-Index - 57
eISSN - 1938-2650
pISSN - 0001-5547
DOI - 10.1159/000521675
Subject(s) - medicine , urine cytology , cytology , urinary system , staining , pathology , urology , podocyte , cystoscopy , proteinuria , kidney
This study investigated whether our urinary podocyte detection method using podocalyxin (PDX) and Wilms tumor 1 (WT1) immunoenzyme staining combined with liquid-based cytology can serve as a noninvasive routine laboratory test for glomerular disease. Methods: The presence of PDX- and WT1-positive cells was investigated in 79 patients with glomerular disease and 51 patients with nonglomerular disease. Results: The frequencies and numbers of PDX- and WT1-positive cells were significantly higher in the glomerular disease group than in the nonglomerular disease group. The best cutoffs for PDX- and WT1-positive cell counts for identifying patients with glomerular disease were 3.5 (sensitivity = 67.1% and specificity = 100%) and 1.2 cells/10 mL (sensitivity = 43.0% and specificity = 100%), respectively. Conclusion: Because our urinary podocyte detection method using PDX immunoenzyme staining can be standardized and it detected glomerular disease with high accuracy, it can likely serve as a noninvasive routine laboratory test for various glomerular diseases.