Open AccessSleep Deprivation Interferes with JAK/STAT Signaling Pathway and Myogenesis in the Masseter Muscle of Rats
Author(s) -
Gomes Galvani Marina,
Moreira Antunes Hanna Karen,
Monico-Neto Marcos,
Yujra Veronica Quispe,
Prado Carla Maximo,
Quintana Hananiah Tardivo,
de Oliveira Flavia,
Ribeiro Daniel Araki
Publication year - 2021
Publication title -
medical principles and practice
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.426
H-Index - 45
eISSN - 1423-0151
pISSN - 1011-7571
DOI - 10.1159/000515307
Subject(s) - original paper
Objectives: The aim of the study was to study the Janus kinase/tyrosine kinase-activated transduction factor (JAK/STAT) signaling pathway and myogenesis on the masseter muscle after sleep deprivation and to investigate the role of stress in this scenario. Subjects and Methods: A total of 18 male Wistar rats were divided into the following groups: control ( n = 6): animals were not submitted to any procedures, and paradoxical sleep deprivation and vehicle (PSD + V; n = 6): animals were subjected to PSD for 96 h and (PSD + MET; n = 6): animals were subjected to PSD for 96 h with administration of metyrapone. Paradoxical sleep deprivation was performed by the modified multiple platforms method. Histopathological analysis, histomorphometry, and immunohistochemistry were performed. Results: The results showed the presence of inflammatory infiltrate in the PSD + V and PSD + MET groups and atrophy. Histomorphometry showed that the cellular profile area decreased, while cellular density increased in both experimental groups. Expression of p-STAT 3, MyoD, and MyoG increased in the PSD + V group, while the PSD + MET group showed increased expression of IL-6 and p-STAT 3. Conclusion: Our results suggest that sleep deprivation induces an inflammatory response and atrophy in the masseter muscle of rats.