Open AccessPostoperative Rise of Circulating Mitochondrial DNA Is Associated with Inflammatory Response in Patients following Pancreaticoduodenectomy
Author(s) -
Niv Pencovich,
Nadav Nevo,
Roi Weiser,
Ekaterina Bonder,
Yoel Bogoch,
Ido Nachmany
Publication year - 2021
Publication title -
european surgical research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.658
H-Index - 46
eISSN - 1421-9921
pISSN - 0014-312X
DOI - 10.1159/000514661
Subject(s) - pancreaticoduodenectomy , mitochondrial dna , medicine , gastroenterology , systemic inflammatory response syndrome , white blood cell , surgery , endocrinology , anesthesia , biology , gene , pancreas , sepsis , biochemistry
Accumulation of plasma mitochondrial DNA (mtDNA) following severe trauma has been shown to correlate with the development of systemic inflammatory response syndrome (SIRS) and may predict mortality. Our objective was to investigate the relationship between levels of circulatory mtDNA following pancreaticoduodenectomy (PD) and the postoperative course. Methods: Levels of plasma mtDNA were assessed by real-time PCR of the mitochondrial genes ND1 and COX3 in 23 consecutive patients who underwent PD 1 day prior to surgery, within 8 h after surgery, and on postoperative day (POD)1 and POD5. The abundance of mtDNA was assessed relative to preoperative levels and in relation to parameters reflecting the postoperative clinical course. Results: When pooled for all patients, the circulating mtDNA levels were significantly increased after surgery. However, while a significant (at least >2-fold and up to >20-fold) rise was noted in 11 patients, no change in mtDNA levels was noted in the other 12 following surgery. Postoperative rise in circulating mtDNA was associated with an increased rate of postoperative fever until day 5, decreased hemoglobin and albumin levels, and increased white blood cell counts. These patients also suffered from increased rates of delayed gastric emptying. No significant differences were demonstrated in other postoperative parameters. Conclusion: Circulating mtDNA surge is associated with an inflammatory response following PD and may potentially be used as an early marker for postoperative course. Studies of larger patient cohorts are warranted.