Open AccessPromoter Methylation of LEP and LEPR before and after Bariatric Surgery: A Cross-Sectional Study
Author(s) -
Wilhelm Julia,
Birkenstock Anna,
Buchholz Vanessa,
Müller Astrid,
Aly Sherif Adel,
Gruner-Labitzke Kerstin,
Koehler Hinrich,
Lichtinghagen Ralf,
Jahn Kirsten,
Groh Adrian,
Kahl Kai G.,
de Zwaan Martina,
Hillemacher Thomas,
Bleich Stefan,
Frieling Helge
Publication year - 2021
Publication title -
obesity facts
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.398
H-Index - 45
eISSN - 1662-4033
pISSN - 1662-4025
DOI - 10.1159/000511918
Subject(s) - research article
DNA methylation constitutes one important epigenetic mechanism that regulates gene expression in human cells. With regard to obesity, bariatric surgery-induced weight loss has been associated with promoter methylation changes in several genes. Hyperleptinemia is a characteristic feature of obesity. The underlying regulating mechanisms have not yet been completely elucidated. Methods: We investigated the methylation of the promoters of the leptin gene ( LEP ) and the leptin receptor gene ( LEPR ) as well as leptin expression in pre- and postbariatric surgery patients using a comparative cross-sectional design. Results: Our results revealed significantly higher LEP promoter methylation patterns in prebariatric surgery patients compared to postoperatively. DNA methylation of the LEPR promoter was significantly higher in the postoperative group. Moreover, we found significantly higher leptin serum levels in patients before the bariatric surgery than afterwards. Discussion: These findings strengthen the suggestion that there is an association between LEP expression and LEP methylation in obesity. We suggest that the epigenetic profile of LEP might be influenced by leptin serum levels in the form of a regulating feedback mechanism.